Chien-Cheng Liu, Yong-Jing Gao, Hao Luo, Temugin Berta, Zhen-Zhong Xu, Ru-Rong Ji & Ping-Heng Tan. Interferon alpha inhibits spinal cord synaptic and nociceptive transmission via neuronal-glial interactions. Scientific Reports 6, Article number: 34356 (2016). doi:10.1038/srep34356

...Immunohistochemistry
Animals were terminally anesthetized with isoflurane and perfused through the ascending aorta with saline followed by 4% paraformaldehyde with 1.5% picric acid in 0.16 M PB. After the perfusion the L4-L5 spinal cord segments and DRGs were collected and postfixed in the same fixative overnight. Spinal cord sections (30 μm, free-floating sections) and DRG sections (20 μm) were cut in a cryostat and processed for immunofluorescence. Tissue sections were blocked with 2% goat serum, and incubated over night at 4 °C with the following primary antibodies: IFN-α/βRβ (rabbit, 1:500, Santa Cruz, sc-30015, M-300), IFN-α antibody (rabbit, R&D, 1:1000), GFAP antibody (mouse, 1:5000, Chemicon), OX-42 antibody (mouse, 1:5000, Serotec), NeuN antibody (mouse, 1:5000, Chemicon), CGRP antibody (chicken, 1:2000, Neuromics), substance P antibody (guinea pig, 1:500, Neuromics), NF-200 antibody (mouse, 1:2000, Sigma), NK-1 antibody (rabbit, 1:2000; Neuromics) and phosphorylated ERK antibody (pERK1/2, rabbit, Cell Signaling, 1:500), as well as FITC-conjugated IB4 (1:200, Sigma). The sections were then incubated for 1 h at room temperature with Cy3- or FITC- conjugated secondary antibodies (1:300, Jackson immunolab). For double immunofluorescence, sections were incubated with a mixture of polyclonal and monoclonal primary antibodies followed by a mixture of FITC- and CY3-congugated secondary antibodies...

RO Cook, PL Durham. Method of Using Dihydroergotamine for Pre-emptive Treatment of Migraine. US Patent 20,150,023,888, 2015

...CGRP (Neuromics, 1:500...

Abeer W Saeed, Alfredo Ribeiro-da-Silva. Non-peptidergic primary afferents are presynaptic to neurokinin-1 receptor immunoreactive lamina I projection neurons in rat spinal cord. Molecular Pain 2012, 8:64 doi:10.1186/1744-8069-8-64.

...rabbit anti-CGRP and guinea pig anti-P2X3 at a 1:25,000 dilution (Neuromics, Edina, MN)...

Tong Liu, Temugin Berta, Zhen-Zhong Xu,Chul-Kyu Park,Ling Zhang, Ning Lü, Qin Liu, Yang Liu, Yong-Jing Gao, Yen-Chin Liu, Qiufu Ma, Xinzhong Dong, and Ru-Rong Ji. TLR3 deficiency impairs spinal cord synaptic transmission, central sensitization, and pruritus in mice. J Clin Invest. 2012 June 1; 122(6): 2195–2207. Published online 2012 May 8. doi:10.1172/JCI45414.

...guinea pig anti-TRPV1 antibody (1:1,000; Neuromics), guinea pig anti-SP antibody (1:1,000; Neuromics), rabbit anti-CGRP antibody (1 2,000; Neuromics)...

Thomas Volckaert, Erik Dill, Alice Campbell, Caterina Tiozzo, Susan Majka, Saverio Bellusci, and Stijn P. De Langhe. Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. J Clin Invest. 2011;121(11):4409–4419. doi:10.1172/JCI58097.

...chicken anti-CGRP (Neuromics)...

Ryan J. Horvathab, Alfonso Romero-Sandoval, Joyce A. De Leo. Inhibition of microglial P2X4 receptors attenuates morphine tolerance, Iba1, GFAP and μ opioid receptor protein expression while enhancing perivascular microglial ED2. PAIN®. Pages 401-413 (September 2010).

...μ opioid receptor (1:5000, Neuromics), CGRP (1:500, Neuromics)...

Hua Zhang and A. S. Verkman. Aquaporin-1 Tunes Pain Perception by Interaction with Nav1.8 Na+ Channels in Dorsal Root Ganglion Neurons. February 19, 2010 The Journal of Biological Chemistry, 285, 5896-5906.

...chicken anti-calcitonin gene-related peptide (CGRP; 1:500, Neuromics, Edina, MN)...

Yong-Jing Gao, Ling Zhang, Omar Abdel Samad, Marc R. Suter, Kawasaki Yasuhiko, Zhen-Zhong Xu, Jong-Yeon Park, Anne-Li Lind, Qiufu Ma, and Ru-Rong Ji. JNK-Induced MCP-1 Production in Spinal Cord Astrocytes Contributes to Central Sensitization and Neuropathic Pain. The Journal of Neuroscience, April 1, 2009, 29(13):4096-4108; doi:10.1523/JNEUROSCI.3623-08.2009.

...calcitonin gene-related peptide (CGRP) antibody (chicken, 1:500; Neuromics)... 

Marcie J. Abbey, Vinit V. Patil, Carrie V. Vause, Paul L. Durham. Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract. Journal of Ethnopharmacology 115 (2008) 238–248.

...Dissociated trigeminal ganglion cells were plated directly on PDL (MW 30,000–70,000; Sigma)-coated 13-mm plastic coverslips equivalent to one ganglion per three coverslips in L15 complete medium. All cells were incubated for 48 h at 37oC then rinsed briefly with phosphate buffered saline (PBS) and treated with 4% paraformaldehyde for 30 min at room temperature and with 0.2% Triton X-100 in PBS for an additional 15 min to fix and permeabilize the cells. Cultures were incubated overnight at 4oC with chicken anti-CGRP antibodies (1:500 in PBS; Neuromics, Edina, MN) or rabbit anti-VR1 N-Terminus (TRPV1) antibodies (1:1000 in PBS; Neuromics). Immunoreactive proteins were detected following incubation with Rhodamine Red X-conjugated donkey anti-chicken or FITC-conjugated donkey anti-rabbit IgG polyclonal antibodies (1:100 in PBS, Jackson Immuno-Research Laboratories, West Grove, PA) for 1 h at room temperature. Prior to viewing, cells were mounted using Vectashield mounting media (Vector Laboratories, Burlingame, CA) containing 4',6 diamidino-2-phenylindole (DAPI) to identify nuclei. Cells were photographed with an Olympus DP70 camera mounted on an Olympus BX41 fluorescent microscope and image collection and analysis performed using Olympus MicroSuite Five imageprocessing software (Center Valley, PA)... 

Dongsheng Wu, Wenlong Huang, Peter M. Richardson, John V. Priestley and Min Liu. TRPC4 IN RAT DORSAL ROOT GANGLION NEURONS IS INCREASED AFTER NERVE INJURY AND IS NECESSARY FOR NEURITE OUTGROWTH. J. Biol. Chem, 10.1074/jbc.M703177200

...Slides were also stained with mouse anti-200-kDa neurofilament (N52 clone, 1:5000, Sigma, Poole, U.K.) and rabbit anti-P2X3 (1:1000, Neuromics) or rabbit anti-CGRP (1:1000) antibodies to visualize distinct DRG subpopulations.


...Chicken anti-CGRP (1:500; Neuromics)...