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MO22147100 ul$295.00Buy Now | Add to Cart
Type: Mouse IgG
Applications: ICC; IF; IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: B; Ch; H; M; P; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
Immunogen: Full-length recombinant human Muscleblind-like 1 protein
IFC Image of Muscleblind-like Protein

Muscleblind-like Protein (MBNL1) mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5-YGCU(U/G)Y-3consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Image: HeLa cells were stained with monoclonal antibody Muscleblind-like protein (MO22147) at 1: 1,000. Muscleblind-like protein 1 is present in the nucleus of HeLa cells (red). Co-staining is with our rabbit polyclonal antibody against vimentin (RA22124, in red) reveals cytoplasmic intermediate filaments.  Blue is a DNA stain which largely colocalizes with the MBNL1 protein in the nucleus.


Diseases associated with MBNL1 include myotonic dystrophy and myotonic dystrophy 1. Among its related pathways are Adipogenesis. GO annotations related to this gene include poly(A) RNA binding and double-stranded RNA binding

Image: Western blot analysis of MO22147. Blot of HL60 cell lysate was probed with MO22147 at dilution 1:1,000. The MO22147 monoclonal binds Muscleblind-like protein 1 at ~40kDa, as expected.