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|MO22147||100 ul||$295.00||Buy Now | Add to Cart|
Muscleblind-like Protein (MBNL1) mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5-YGCU(U/G)Y-3consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
Image: HeLa cells were stained with monoclonal antibody Muscleblind-like protein (MO22147) at 1: 1,000. Muscleblind-like protein 1 is present in the nucleus of HeLa cells (red). Co-staining is with our rabbit polyclonal antibody against vimentin (RA22124, in red) reveals cytoplasmic intermediate filaments. Blue is a DNA stain which largely colocalizes with the MBNL1 protein in the nucleus.
Diseases associated with MBNL1 include myotonic dystrophy and myotonic dystrophy 1. Among its related pathways are Adipogenesis. GO annotations related to this gene include poly(A) RNA binding and double-stranded RNA binding
Image: Western blot analysis of MO22147. Blot of HL60 cell lysate was probed with MO22147 at dilution 1:1,000. The MO22147 monoclonal binds Muscleblind-like protein 1 at ~40kDa, as expected.