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MO22148100 ul$295.00Buy Now | Add to Cart
 
Type: Mouse IgG
 
Applications: IF; IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Description/Data:
DJ1/Park7 Antibody IHC Image

DJ-1, also known as Park7, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ1 superfamily that functions as protein chaperones, catalases, proteases and kinases. DJ-1 was originally cloned as an oncogene that cooperatively transforms cells together with H-ras. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease. DJ-1 may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke

Image: HeLa cells stained with MO22148 (green), and CH22108 chicken antibody to vimentin (red) and DNA (blue). MO22148 antibody reveals strong cytoplasmic staining for DJ-1 in human cells, but does not bind to rodent cells

 

WB DJ1/PARK7

Image: Blot of 20 µg rat brain lysate (lane 1) and 20 µg HeLa cell lysate (lane 2) was probed with MO22148 antibody to DJ-1at 1:5,000. Notes that the strong single band running at about 21kDa corresponds to DJ-1 in HeLa cells, while DJ-1 is barely seen in rat brain lysate. This antibody binds also to bovine DJ-1 on western blots but does not recognize rat or mouse DJ-1.