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Type: Goat IgG
 
Applications: ICC; IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Recombinant human caspase-9, aa 139 - 330
 
Description/Data:
Image of Caspase-9 antibody, western blot

Caspase-9 is a cytoplasmic proenzyme that associates with both pro-apoptotic and anti-apoptotic proteins. It is a key regulator of apoptosis and also functions in embryonic development.

Also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.

Image: Immunoblots of extracts from 2 x 105 human Jurkat cells treated with staurosporine (STS, 1 μg/mL) for the indicated times. Samples were electrophoresed on 15% gels and immunoblotting was with 1.0 μg/mL goat anti-Caspase-9 that detects the precursor and 34 and 37 kDa forms of pro + LSU.

 

Other Reagents to Consider:

Antibodies:

Caspase-3

Caspase-3, active

Caspase-7

Caspase-9

Caspase-10b

Caspase-12

Bcl-2

Vimentin-Catalog#: CH230101

Vimentin-Catalog# CH22108

Apoptosis Antibodies

Caspases and Cathepsins

Cancer Antibodies

Neurodegenerative Disease

Proteins

Bcl-2

 

All Apoptosis Reagents

 

 

Stem Cell Reagents