Omi/HtrA2

Mouse Monoclonal Antibody
DATASHEET(pdf - 42Kb)
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Type: Mouse IgG
 
Applications: IHC; WB
ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; WB=Western blotting; FC=Flow Cytometry; IP=Immunoprecipitation; E=ELISA; NB=Neutralization of Bioactivity; FACS; FM=Fluorescent Micsroscopy; ; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; ; ; AC=Adherent Cell Assays; ; ; NAC=Non-adherent Cell Assays; ; ; BSC-CM5= Biacore Sensor Chip CM5; ;
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins
Format: Protein G Purified - liquid
 
Immunogen: Hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human High Temperature Requirement Protein-2 (rhOmi/HtrA2;aa 134 - 458) with a carboxylterminal six histidine tag (GenBank accession # AF141305).
 
Description/Data:
Picture

The mammalian serine protease Omi (also known as HtrA2), was identified as a 49 kDa serine protease, homologous to the bacterial endoprotease HtrA (high-temperature requirement). It is a homotrimeric serine protease that localizes to the mitochondria and is processed to expose an N-terminal reaper-like motif similar to SMAC/Diablo. Omi/HTRA2 is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition.

It is suspected to play a role in Parkinson's, Alzheimer's, Diabetes and certain forms of cancer.

Image: Detection of HtrA2/Omi with MAB1458.Lysates of human SK-MEL-28, MCF-7, HEK293 and HeLa cells were resolved by SDS-PAGE, transferred to Immobilon-P membrane and immunoblotted with 1.0 g/mL monoclonal anti-HtrA2/Omi, as described in Protocols for Immunoblotting. A 45 second exposure to film is shown

Other Reagents to Consider:

Apoptosis

Neurodegenerative Disease

Diabetes and Obesity

Cancer