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GT15194100 ug$375.00Buy Now | Add to Cart
 
Type: Goat IgG
 
Applications: IHC; WB; IP
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Recombinant mouse Bcl-2, aa 1 - 208, which lacks the C-terminal mitochondrial targeting sequence. Immunogen was tagged with 6X-histidines at the C-terminal.
 
Description/Data:
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This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl 2, such as in the case of translocation of Bcl 2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. The Bcl-2 gene has been implicated in a number of other cancers and diseases including:  melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity. It is also thought to be involved in resistance to conventional cancer treatment. This supports a role for decreased apoptosis in the pathogenesis of cancer

IHC Image: Bcl-2 staining of  human breast cancer tissue. Cells were stained using ABC-HRP + NovaRed (red) substrate and Haematoxylin (blue) counterstain. . Nakopoulou, L. et al., Histopathology, 1999, 34(4):310-319.

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WB Image (right): SDS extracts from 3 x 106  human KGI and 1 x 106 mouse MNFS60 cells were also electrophoresed on the gel. After transfer to membranes, the samples were blotted with anti-Bcl-2. Methods are described in the attached protocol.

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IP Image (left): SDS extracts from 3 x 106 human KGI and 1 x 106 mouse MNFS60 cells were also electrophoresed on the gel. After transfer to membranes, the samples were blotted with anti-Bcl-2.