Catalog NumberSizePrice (USD)Shopping Cart
GT41007100 ug$310.00Buy Now | Add to Cart
P41007100 ug Blocking Peptide$145.00Buy Now | Add to Cart
 
Type: Goat IgG
 
Applications: IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Synthetic peptide: C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1.
 
Description/Data:
Picture

This gene encodes the largest subunit of dynactin (DCTN), a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. 

Image:DCTN1 staining  (dilution: 2.5µg/ml) staining of human brain tissue, cortex (formalin-fixed paraffin-embedded) followed by biotinylated horse anti-goat IgG secondary antibody,  alkaline phosphatase-streptavidin and chromogen. Protocol on data-sheet.

Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy. Defects in DCTN1 are the cause of Perry syndrome (PERRYS) also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss.

Picture[2]

Image: DCTN1 staining (dilution: 1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.