|Catalog Number||Size||Price (USD)||Shopping Cart|
|RA22104||100 ul||$275.00||Buy Now | Add to Cart|
Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
Image: Mixed neuron/glia cultures from newborn rat brain stained with antibody to peripherin (green) and rabbit polyclonal antibody NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA. Protocol on Data-sheet.
NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease and Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease.
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes by Category
Image: Western blot of whole rat cerebellum homogenate stained NF-L in the left strip, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rodent NF-L. Faint bands below this are in vivo degradation products generated by calpain digestion. The major NF-H band in human, cow and bovine run a little slower on SDS-PAGE, at about 70kDa. Right lane shows a strip incubated with a new antibody against Neuron Specific Enolase, which reveals a major band at 47kDa. Protocol on Data-sheet.