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Type: Mouse IgG
Applications: IF; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
Immunogen: Purified, E. coli-derived, recombinant human 14-3-3 eta protein.

The 14.3.3 family of proteins were originally discovered as prominent protein spots on 2 dimensional gels. They are a family of 28-33kDa proteins which are often major proteins of the cytoplasm (1). They function as binding partners for phosphoserine and phosphthreonine sites in other proteins, though they have other binding partners which are not dependent on phosphorylation. These binding interactions are important in the regulation of molecules such as the signaling kinases in the MAP kinase pathway c-Raf and b-Raf, the proapoptotic molecules Bad and Bax and the cell cycle regulator Cdc25.

Image: rat mixed neuron/glial cultures stained with 14-3-3 eta antibody (red). Neuronal perikarya are very rich in 14-3-3 eta which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain. Protocol on data-sheet.

The 14.3.3 eta protein accumulates in the CSF of patients suffering form Creutzfeld Jacob Disease, and can be used for the diagnosis of this disease. Furthermore, this protein binds α-synuclein in the Lewy bodies of Parkinson's disease and has been linked to early-onset schizopherenia . The HGNC name for this protein is YWHAH.


Image: Blots of crude HeLa cell homogenate blotted with 14-3-3 eta (left lane) and various molecular weight standards (right lane- numbers indicate apparent SDS-PAGE molecular weight in kDa). The mouse monoclonal antibody binds strongly and cleanly to a band at about 28kDa. Protocol on Datasheet.