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Type: Mouse IgG
 
Applications: IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Protein G Purified - liquid
 
Immunogen: Hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, insect cell line Sf 21-derived, recombinant human insulin-like growth factor I receptor (rhIGF-I R) extracellular domain.
 
Description/Data:
Picture

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues.

Images: (1) Lysates were prepared from NTERA-2 human embryonal carcinoma cells, SK-MEL-28 human malignant melanoma cells, and G-361 human malignant melanoma cells in non-reducing sample buffer. The lysates were resolved by SDS-PAGE and transferred to an Immobilon-P membrane. The blot was developed with 1 μg/mL monoclonal anti-human IGF-I R antibody and WesternGlo Chemiluminescent Detection Substrate. (2) IGF-I R (at a dilution of 25 µg/mL) staining of paraffin-embedded human skin tissue. Tissue stained with anti-mouse HRP-DAB (Brown) and counterstained with haematoxylin (blue).