Cyclooxygenase (COX), also known as prostaglandin G/H synthase, is a membrane bound enzyme partly responsible for the catalysis of prostanoid synthesis. COX is expressed as at least three different isoforms. COX-1 is constitutively expressed and thought to regulate a number of 'housekeeping' functions such as vascular hemostasis, renal blood flow, and glomerular function. COX-2 expression is tightly regulated and induced by inflammatory mediators such as growth factors, cytokines, and endotoxin. COX-3 appears to be much more strictly regulated spatially and is observed in greatest abundance in cerebral cortex.
Image: Cox-2 Western Blots. Lysates from human peripheral blood mononuclear cells (PBMCs) and mouse RAW.264 cells, untreated or treated with LPS and human U937, untreated or treated with PMA and LPS were resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1.0 μg/mL goat anti-COX2