|Catalog Number||Size||Price (USD)||Shopping Cart|
|GP10106||50 ul||$229.00||Buy Now | Add to Cart|
|GP10106||100 ul||$379.00||Buy Now | Add to Cart|
|P10104||50 ul Blocking Peptide @ 2mgs/ml||$95.00||Buy Now | Add to Cart|
Three types of opioid receptors have been cloned -- mu, delta, and kappa. Opioid receptors are seven transmembrane G-protein coupled receptors. They share a high degree of homology and are most divergent at the N- and C-termini. Activation of mu opioid receptors leads to a decrease in neuronal excitability.
Image: Mu Opioid Receptor staining of Rat Dorsal Horn. Courtesy of Dr. Louis Gendron, University of Sherbrooke.
Rats were deeply anesthetized with isoflurane and perfused through the aortic arch with 100 ml of heparin (75 U/ml of heparin in 0.9% saline) followed by 100 ml of a mixture of 3.75% acrolein and 2% PFA in 0.1 M PB, pH 7.4, and then by 300 ml of 2% PFA in the same buffer at 45 ml/min.m Lumbar spinal cord was removed and postfixed in 2% PFA in 0.1 M PB for 1 h at 4°C. Sections (50 µm thick) were cut using a Vibratome and processed for MOR labeling.
Sections were incubated in 1% sodium borohydride for 30 min and extensively rinsed in 0.1 M PB. They were then cryoprotected for 30 min in a solution consisting of 25% sucrose and 3% glycerol in 0.05 M PB and snap frozen with isopentane (–50°C) followed by liquid nitrogen.
After being rapidly thawed in 0.1 M PB, sections were rinsed with TBS 0.1M and preincubated for 1 h at room temperature in 3% NGS diluted in TBS. They were then incubated for 36 h at 4°C in MOR antiserum diluted 1/500 in TBS containing 0.5% NGS. Sections were then rinsed twice with TBS and incubated for 1 h at room temperature with biotinylated anti-guinea pig antibody (1/400; Vector Laboratories).
Following three 10 min washes in TBS, sections were incubated 30 min with Vectastain Elite ABC (Vector Laboratories). Sections were rinsed three times with TBS and peroxidase complex revealed for 8 minutes with DAB substrate (2.2 mg/10 ml + 0.01% H2O2).
At the end of this incubation, sections were washed twice with TBS, mounted on microscope slides, and dehydrated with ethanol.
Neurotransmissiom Research Antibodies
i-Fect Transfection Kit
gene silencing of DOR, NaV1.8 tetrodotoxin-resistant sodium channel, NTS2 and more in-vitro and in vivo
WB Images: Representative Western blots of isolated mouse brain striatum and cerebellum for the non-phospho-MOR antibody or the MOR-P2 antibody. The samples from cerebellum demonstrate a lack of IR for both the non-phospho-MOR antibody and MOR-P2 antibody further showing the selectivity of these antibodies. The images are each representative of four independent experiments. Dilution 1:1000. Neuroscience 146 (2007) 1795–1807.