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P10109100 ug Blocking Peptide$95.00Buy Now | Add to Cart
Type: Guinea Pig IgG
Applications: ICC; IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Whole serum - liquid

P2X2 is a subunit in the family of ion channels activated by extracellular ATP. It was cloned from PC12 cells. P2X2 has two putative transmembrane domains with intracellular N- and C-termini. It can form homomeric channels and heteromeric channels with other members of the family. P2X2 is expressed in brain, spinal cord, sensory and autonomic ganglia as well as in neuroendocrine cells.

Image: P2x2 staining of Cerebellar Purjinke cell layer. ~50 μm sections from 3 brains from P15 rats.;1/41/ra8/DC1/1

P2X2: Customer Publications


Images: The constitutive interaction between P2X2 receptors and VILIP1 is calcium independent. Calcium dependence of the interaction between P2X2 receptors and VILIP1 was assessed by coimmunoprecipitation from HEK cells expressing P2X2-FLAG receptors and either VILIP1or VILIP1 EF-hand mutants. The effect of calcium on the interaction was tested before cell lysis by increasing intracellular calcium with ATP application or ionomycin treatment, and also during the immunoprecipitation steps. In the latter case, experiments were carried in the absence of calcium and in the presence of 5 mM EDTA or in the absence of EDTA and in the presence of 500μM calcium. P2X2 receptors were immunoprecipitated using a FLAG antibody conjugated to agarose beads. Bound proteins were eluted by competition using a FLAG peptide. As shown in the top panel, no difference in P2X2-FLAG-VILIP1 interaction was observed in the presence or the absence of calcium in the immunoprecipitation buffer. In addition, stimulation of transfected cells with ATP (100μM, 5 minutes) or with ionomycin (2μM, 5 minutes) prior to lysis did not affect the interaction between the two proteins. A similar observation was made when the VILIP1 EF hand mutant was co-immunoprecipitated. No immunoprecipitation was observed when VILIP1 was transfected alone. The bottom panel shows the total protein input used for immunoprecipitation.