Catalog NumberSizePrice (USD)Shopping Cart
GT41012100 ug$310.00Buy Now | Add to Cart
P41012100 ug Blocking Peptide$145.00Buy Now | Add to Cart
 
Type: Goat IgG
 
Applications: IHC; WB; E
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: B; H; M; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Synthetic peptide: C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3; NP_001070711.1; NP_001070712.1.
 
Description/Data:
Picture

Human glutamate receptor 4 (GRIA4) is a new member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. Binding studies showed that human GluR4 transfected into simian kidney cells (COS-1) exhibits high specific binding for[3H](RS)-alpha-amino- 3-hydroxy-5-methylisoxazole-4-propionic acid ([3H]AMPA) but not [3H]kainate. Ion substitution experiments indicate that hGluR4 receptor-linked ion channels in their homomeric state are permeable to both CA2+ and Na+ ions. Immunoprecipitation studies suggest that GRIA1 exists in situ in the form of a pentamer.

Image: GluR4 staining of human brain tissue, cortex (formalin-fixed paraffin-embedded) at 5 ug/ml followed by biotinylated horse anti-goat IgG secondary antibody,  alkaline phosphatase-streptavidin and chromogen. Protocol on data-sheet.

Nociceptors, involved primarily in the mediation of neuropathic pain, may be presynaptically modulated by GluR4-containing AMPA receptors.

Picture[2]

Image: GluR4 (0.3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.