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GT15074100 ug$365.00Buy Now | Add to Cart
 
Type: Goat IgG
 
Applications: IHC; WB; E; NB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Recombinant chicken transforming growth factor beta 3
 
Description/Data:
Image of TGFB3 IHC Staining of Human Brain Tissue

Transforming Growth Factor beta 3 (TGF beta 3) is a member of the TGF beta family of growth factors. The TGF beta polypeptides are multifunctional, capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. The multi-modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide

Image: Paraffin-embedded tissue sections of human brain stained with anti-TGF-beta3. HRP-DAB (brown)shown TGF-beta3 immunoreactivity and Haematoxylin (blue) counterstain.

Image of TGF Beta 3 Competitive Binding Assay

Neutralization of TGF-β3 bioactivity

Figure 1: TGF-β3 inhibits the mIL-4-dependent 3H-thymidine incorporation by HT-2 cells in a dose-dependent manner (Tsang, M. et al., 1995, Cytokine 7:389). The ED50 for this effect is typically 0.01 - 0.03 ng/mL.

Figure 2: To measure the ability of the antibody to neutralize the bioactivity of TGF-β3 on HT-2 cells, rhTGF-β3 was incubated with various concentrations of the antibody for 1 hour at room temperature in a 96 well microtiter plate. Following this preincubation period, HT-2 cells were added. The assay mixture in a total volume of 100 μL/well, containing antibody at the concentrations indicated, rmIL-4 at a concentration of 7.5 ng/mL, rhTGF-β3 at a concentration of 0.1 ng/mL, and cells at 1 x 105 cells/mL, was incubated at 37° C for 48 hours in a humidified incubator. 3H-thymidine was added during the final four hours. The cells were subsequently harvested onto glass fiber filters and the 3H-thymidine incorporated into DNA was determined. The ND50 of the antibody, under these conditions, is approximately 0.005 - 0.015 μg/mL.