GAD1/GAD67

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter γ-amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.  

GAD1/GAD67: Customer Publications

Image: Detection of GAD1/GAD67 in paraffin-embedded human cerebellum tissue sections at a dilution of 15 µg/mL. Tissues were stained with anti-goat HRP-DAB (brown) and counterstained with hematoxylin (blue). 

Image: GAD1 staining of inhibitory neurons in the mouse cortex. Neurons were stained with  antigoat alexafluor 568 secondary from invitrogen(red) and a dapi counterstain (blue). Courtesy of Dr. Aaron Hirko, University of Pittsburgh.