Leptin (Human)

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

Image: Leptin stsining of paraffin-embedded human brainsections. Tissue was stained

with the anti-mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).

Leptin (Human) Customer Publications

Figure 1: rhLeptin stimulates the 3H-thymidine incorporation by mouse BAF/Leptin R cells in a dose-dependent manner. The ED50 for this effect is typically 0.4 - 2 ng/mL.

Figure 2: Approximately 0.4 - 1.6 μg/mL of the antibody will neutralize 50% of the bioactivity due to 7.5 ng/mL of rhLeptin.