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Type: Rabbit IgG
 
Applications: ICC; IF
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Immunogen: Synthetic Leu5-enkephalin (H-Tyr-Gly-Gly-Phe-Leu-OH) conjugated through N-terminal tyrosine to BSA.
 
Description/Data:
Picture

leu-enkephalin (tyr-gly-gly-phe-leu) is a pentapeptide which compete with and mimic the effects of opiate drugs. It is involved in modulation of nociceptive pain.

Cross-reactivity (%)

  • Leu5-Enkephalin                  100
  • Met5-Enkephalin                   0.93
  • beta-Endorphin                     0.01
  • beta-Lipotropin                     0.01

Image: Immunofluorescent detection of Leu-Enkephalin in rat spinal cord dorsal horn (red color). 

References 

1.        Elde R, Hokfelt T, Johansson O, Terenius L. (1976) Neuroscince, 1:349-351.
2.        Stengaard-Pedersen K, Larsson LI. (1981) Histochemistry, 73(1):89-114.
3.        Palkovits M. (1984) Progress in Neurobiology, 23:151-189.
4.        Kalivas PW. (1985 ) Neuroscience & Biobehavioral Reviews, 9(4):573-587.
5.        Wang BL, Larsson LI. (1985) Histochemistry, 83(1):47-56.
6.        Moore MR, Black PM. (1991) Neurosurgical Review, 14(2):97-110.