Nestin

Mouse Monoclonal Antibody
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Type: Mouse IgG
 
Applications: ICC; IHC
E=ELISA; FC=Flow Cytometry; ICC=Immunocytochemistry; IF=Immunofluorescence; IP=Immunoprecipitation; IHC=Immunohistochemistry; SE=Sandwich ELISA; WB=Western blotting; NB=Neutralization of Bioactivity; FACS; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; BSM=Biosactive Small Molecule or Peptide; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; AC=Adherent Cell Assays; NAC=Non-adherent Cell Assays; CDM=Cell Differentiation Media; BSC-CM5= Biacore Sensor Chip CM5; FM=Fluorescent Micsroscopy; ; ; ; ; ; ; ; ; ;
Species Reactivity: M; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Protein G Purified - liquid
 
Immunogen: Recombinant rat Nestin (a.a. 544-776)
 
Description/Data:

Mouse nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

Nestin Customer Publications

More Links:

Nestin Mouse Monoclonal-Cat#:MO15012

Nestin -Chicken Polyclonal     

Nestin-Goat Polyclonal   

Neural Progenitor Markers                 

Stem Cell Research Proteins

Human Neural Progenitor & Neuron Discovery Kits
 

Picture[3]

 

Images: Immunostaining of  hNP1 Progenitors before and after differentiation. The culture was highly homogenous with neural rosettes. (A) Neural rosettes (white arrows, bright field) from WA09 cells. A similar result also obtained with BG02 cell line. (B) Shown here, a neural rosette stained with Nestin (NES) (green) antibody. Propagated Neural progenitors showed expression of marker genes, NES (C) and Musashi 1 (D) but not SOX2 (E). Further differentiation produced neurons (Tuj1) (F), astrocytes (GFAP) (G) and oligodendrocytes (myelin basic protein)(H) (lower panel). DAPI (blue) was used for staining the nuclei (scale bar for (A) through (E) is 100 mm and for the remaining figures 10 mm). Differentiation (2007) DOI: 10.1111/j.1432-0436.2007.00256.x...Dilutions: NES (1:100, Neuromics, Edina, MN), MSI1 (1:100, Neuromics), Tuj1 (1:500, Neuromics)...