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MO22941100 ul$350.00Buy Now | Add to Cart
Type: Mouse IgM
Applications: IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Whole serum - lyophilized
Immunogen: The TH moAb recognizes an epitope within the catalytically-active C-terminal end-product. This C-term product is ~34kDa. (This end product is the result of a partial digestion of the rat TH with chymotrypsin.)

The histochemical antibody for tyrosine hydroxylase (TH) is a mouse monoclonal antibody generated against TH that has been isolated and purified from rat PC12 cells.  The antibody is believed to have wide species cross-reactivity because it recognizes an epitope in the mid-portion of the TH molecule where extensive species homology exists.

This Tyrosine Hydroxylase antiserum was quality control tested using standard immunohistochemical methods.  The antiserum demonstrates strongly positive labeling of rat catecholamine neuron systems using indirect immunofluorescent and biotin/avidin-HRP techniques.  Recommended primary dilutions for these methods are provided on the data-sheet. This antibody does not cross react with dihydropterdine reductase, dopamine-B-hydroxylase, phenylethanolamine-N-methyltransferase, phenylalanine hydroxylase or tryptophan hydroxylase using Western blot methods.

Image: TH Staining in the Substantia Nigra.

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