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FC15008100 Tests$305.00Buy Now | Add to Cart
Type: Mouse IgG
Applications: FC
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Protein G Purified - liquid
Immunogen: Clone #: 2D1

Leukocyte Common Antigen, CD45, is a transmembrane protein tyrosine phosphatase (1, 2). Isoforms of CD45 are generated by differential glycosylation and by alternative splicing of several exons encoding amino terminal domains of the protein (1, 2). The pattern of expression of different CD45 isoforms is tightly regulated during lymphoid development and is modulated upon antigenic exposure (1, 2). 

The 2D1 antibody is produced by a hybridoma generated from the fusion of mouse NS1 myeloma cells with splenic B cells from a BALB/c mouse immunized subcutaneously with human peripheral blood mononuclear cells (3). This antibody recognizes CD45 molecules highly expressed on T cells, B cells, and medulary thymocytes (4). The 2D1 antibody stains CD45 isoforms weakly expressed on granulocytes, monocytes, and macrophages (4). CD45 is not expressed on platelets or erythrocytes (4). Accordingly, CD45 has been used as a marker for all nucleated cells in the peripheral blood.

Image: Whole blood lymphocytes stained with anti human-CD45-PE (Catalog # FC15008, open histogram) or isotype control ( filled histogram).

1. Trowbridge, I.S. (1991) J. Biol. Chem. 266:23517.
2. Hemler, M.E. et al. (1993) in Leukocyte Typing V, eds. Schlossman, S.F. et al., Oxford University Press, Oxford, pp. 1617 - 1618.
3. Beverley, P. (1980) in Proc. XI. International Course on Transplant and Clinical Immunology, Excerpta Medica, Amsterdam. pp. 87 - 94.
4. Pizzolo, G. et al. (1980) Cancer 46:2640.