Catalog NumberSizePrice (USD)Shopping Cart
RA18032100 ul$325.00Buy Now | Add to Cart
 
Type: Rabbit IgG
 
Applications: WB; IP
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: Ch; H; M; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Synthetic peptide (KLH-coupled) corresponding to (KLH-coupled) corresponding to Leu330 of human TCF4.
 
Description/Data:
Picture

LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of the following: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1/TCF bind to Wnt response elements to provide a docking site for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1/TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).

TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting studies indicate that TCF4 is required to maintain the crypt stem cells of the small intestine (6,7). TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression (8,9). Studies also indicate that a variant of the TCF4 gene confers an increased risk of type 2 diabetes (10).

Image: Western blot analysis of extracts from various cell types using TCF4 (C48H11) Rabbit mAb.

References:
1. Waterman, M.L. (2004) Cancer Metastasis Rev. 23, 41-52.
2. Schilham, M.W. and Clevers, H. (1998) Semin. Immunol. 10, 127-132.
3. Brantjes, H. et al. (2002) Biol. Chem. 383, 255-261.
4. Reya, T. and Clevers, H. (2005) Nature 434, 843-850.
5. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
6. Cho, E.A. and Dressler, G.R. (1998) Mech. Dev. 77, 9-18.
7. Korinek, V. et al. (1998) Nat. Genet. 19, 379-383.
8. Howng, S.L. et al. (2004) Int. J. Oncol. 25, 1685-1692.
9. Shiina, H. et al. (2003) Clin. Cancer Res. 9, 2121-2132.
10. Grant, S.F. et al. (2006) Nat. Genet. 38, 320-323.

Other Reagents to Consider:

TCF4 (C9B9)

Stem Cell Reagents

 

All Cancer Antibodies

 

 

Diabetes/Obesity Antibodies

All Proteins