Type: Goat IgG
Applications: IHC; WB; E
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
Immunogen: Purified, NS0-derived, recombinant mouse Chordin (rmChordin) (aa 27 - 948).
Chordin is an extracellular antagonist of BMP signaling which is thought to readily diffuse in tissues, thus forming gradients of BMP inhibition that result in reciprocal gradients of BMP signaling thus impacting cell fate determination along the embryonic dorsoventral axis.
This antibody will block > 95% of the binding of 100 ng/mL of rhBMP-4 to immobilized rmChordin. (100 μL of 5 g/mL was coated in each well) in a functional ELISA. 50% inhibition occurs at an antibody concentration of approximately 3 - 12 μg/mL.
Image: Chordin staining of cryostat tissue sections of mouse liver. Tissues were stained using Goat (red-brown) and counterstained with hematoxylin (blue).