|Catalog Number||Size||Price (USD)||Shopping Cart|
|GT15052||100 ug||$365.00||Buy Now | Add to Cart|
Oct-3/4 is a 34 kDa POU transcription factor that is expressed in embryonic stem (ES) cells and germ cells, and its expression is required to sustain cell self-renewal and pluripotency. Oct-3/4 is the most recognized marker for totipotent ES cells.
Image: Oct-3/4 Staining in human embryonic stem cells. Cells were stained using Rhodamine Red-conjugated donkey anti-goat secondary antibody.
Right Image: Applying methods and neural markers for sorting of hESC-derived differentiated neuronal cell types: FACS for NCAM for in vitro and in vivo proof-of-principle studies. (a) NCAM was upregulated upon neural differentiation in vitro and clusters of immature, Oct-4+ cells did not express NCAM, allowing for positive selection strategies to eliminate unwanted immature stem cells. Downloaded from www.stemcells.com by Ole Isacson on June 22, 2007
Left Image: Oct-3/4 in Embryonic Stem Cells. Human embryonic stem cells were cultured with recombinant human FGF-2, basic (Catalog #: PR27013) in the presence (left) or absence (right) of Mouse Embryonic Fibroblast(MEF)-conditioned medium. SSEA-4 and Oct-3/4 were detected using anti-human SSEA-4 monoclonal antibody and anti-human Oct-3/4 polyclonal antibody. Cells were stained with Alexa Fluor® 568-conjugated,anti-mouse secondary antibody (SSEA-4; red) and Alexa Fluor 488-conjugated anti-goat secondary antibody. (Oct-3/4; green). Cells were counterstained with DAPI (blue).
Image: IGnT-KO ES cells exhibited properties shared with other ES cells. (A) Phase contrast microphotograph of IGnT-KO ES. IGnT-KO ES cells formed round colonies on SL-10 feeder cells. (B) Immunostaining. The nuclei of D3 or IGnT-KO ES cells were stained with goat anti-Oct 3/4 antibody. (C) RT-PCR. Oct 3/4, Sox-2 and Rex-1 were expressed in both IGnTKO and D3 cells, but IGnT was only expressed in D3. Scale bar; 100 µm. Glycobiology 2008 18(3):242-249; doi:10.1093/glycob/cwm138.