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GT15244100 ug$365.00Buy Now | Add to Cart
Type: Goat IgG
Applications: ICC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
Immunogen: E. coli-derived recombinant human Brg1. Gln673-Asn774

Brg1, also known as ATP-dependent helicase SMARCA4, is a  member of the SNF2 helicase family of molecules. It may  facilitate and inhibit gene transcription. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin.  Brg1 works to activate or repress transcription.  It is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells

It is also thought to be crucial to the development of sperm and smooth muscle in the gastrointestinal tract.

Image: Brg1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody at 10 µg/mL for 3 hours at room temperature. Cells were stained with 557-conjugated anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.  Protocol and data-sheet.


Image: Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, cytoplasmic and nuclear extracts. PVDF membrane was probed with 2 µg/mL Brg1 followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band for Brg1 was detected at approximately 205 kDa (as indicated). Protocol and data-sheet.