|Catalog Number||Size||Price (USD)||Shopping Cart|
|GT15244||100 ug||$365.00||Buy Now | Add to Cart|
Brg1, also known as ATP-dependent helicase SMARCA4, is a member of the SNF2 helicase family of molecules. It may facilitate and inhibit gene transcription. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Brg1 works to activate or repress transcription. It is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells
It is also thought to be crucial to the development of sperm and smooth muscle in the gastrointestinal tract.
Image: Brg1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody at 10 µg/mL for 3 hours at room temperature. Cells were stained with 557-conjugated anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Protocol and data-sheet.
Image: Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, cytoplasmic and nuclear extracts. PVDF membrane was probed with 2 µg/mL Brg1 followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band for Brg1 was detected at approximately 205 kDa (as indicated). Protocol and data-sheet.