Neurofilament NF-H

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Image: Human cerebellar cortex fixed in formalin, embedded in paraffin and stained with Mouse Monoclonal NF-H using the ABC (avidin biotin conjugate) method. The section was counterstained with heamatoxylin-eosin (blue). MCA-NAP4 stains prominent basket cell axons surrounding the large Purkinje neurons. Granule cell layer at bottom of image, molecular layer at top. Protocol on Data-sheet.

Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia. 

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Image: Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including: Mouse Monoclonal NF-H (Lane 2), NF-M, NF-L, NF66 and GFAP. Protocol on Data-sheet.