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Type: Mouse IgG
 
Applications: FC; ICC; IF; IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: Ca; H; M; Pr; R; Rb
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Ascites Fluid - liquid
 
Immunogen: Purified Na+,K+-ATPase from rabbit renal outer medulla.
 
Description/Data:
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is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, wherease the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. This ATPase Na+/K+ transporting alpha 1 is expressed in both neuronal and non-neuronal cells.

Images: Immunohistochemistry: ATPase Na+/K+ alpha 1 staining of enodmetrial glands within the uterus. Note the absence of staining in the surrounding myometrial smooth muscle. Western Blotting: Detection of ATPase Na+/K+ alpha 1 in in porcine proximal tubule protein. Protocols on Datasheet.

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Image: Immunocytochemistry: Detection of ATPA1 (Green) in HepG2 cells using ATPase Na+/K+ alpha 1. Nuclei (Blue) were counterstained using Hoechst 33258.

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Images: Flow Cytometry / FACS analysis: Detection of ATPase Na+/K+ alpha 1 in fixed Hela cells.