|Catalog Number||Size||Price (USD)||Shopping Cart|
|RA22115||100 ul||$295.00||Buy Now | Add to Cart|
GAP-43 (growth associated protein 43 kDa; also known as neuromodulin) is a 24 kDa, neuron growth cone-associated member of the neuromodulin family. Apparently due to its tertiary structure, it runs anomalously at 43 kDa on SDS-polyacrylamide gel. Rat GAP-43 is a 226 amino acid (aa) protein that contains an N-terminal lipid binding motif, an 11 aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa 38 - 48), and a C-terminus with four potential serine phosphorylation sites. The molecule may utilize the IQ domain to bind calmodulin. Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction. Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.
Image . Mixed neuron-glial cultures stained with rabbit antibody to GAP43 (red), blue is DNA staining. Protocol and Data-sheet
Note: This antibody is useful for Western analysis where it recognizes a band at 43 kDa. GAP43 is a proven marker for developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. Can be used with Mouse Monoclonal Antibody GAP43-Catalog#: MO22118 for dual labeling cell cultures and tissue.
Neuronal-Glial Markers-Astrocytes, Glia, Microglia, Olidogodendrocytes, Progenitors and Schwann Cell Markers
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes