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MO22118100 ul$275.00Buy Now | Add to Cart
 
Type: Mouse IgG
 
Applications: ICC; IF; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Supernatant - liquid
 
Immunogen: C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, to which an N-terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
 
Description/Data:
Picture[2]

GAP-43 (growth associated protein 43 kDa; also known as neuromodulin) is a 24 kDa, neuron growth cone-associated member of the neuromodulin family. Apparently due to its tertiary structure, it runs anomalously at 43 kDa on SDS-polyacrylamide gel. Rat GAP-43 is a 226 amino acid (aa) protein that contains an N-terminal lipid binding motif, an 11 aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa 38 - 48), and a C-terminus with four potential serine phosphorylation sites. The molecule may utilize the IQ domain to bind calmodulin. Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction. Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.

Image: E18 hippocampal neurons grown for four days and stained in the green channel with monoclonal antibody to Growth Associated Protein 43 (GAP43) an abundant protein of neuronal membranes especially concentrated in axonal membranes and growth cones. GAP43 is heavily palmitoylated in the Golgi, so this antibody can be used to localize the neuronal Golgi complex. Blue stain is DAPI and reveals cell nuclei of these neuronal cells. Protocol on data-sheet.

Note: This antibody is useful for Western analysis where it recognizes a band at 43 kDa. GAP43 is a proven marker for developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. Can be used with Rabbit Polyclonal GAP43-Catalog#: RA22115 for dual labeling cell cultures and tissue.

Picture[2]

Image: Western blot of whole rat spinal cord lysates probed with mouse monoclonal antibody to GAP43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. Note that the strong single band running at about 43kDa corresponds to GAP43.