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Angiogenesis Antibody Arrays Designed for For Simultaneous Detection of the Relative Levels of up to 43 Angiogensis Related Proteins

Our C-Series Apoptosis Array Kits give you the abilility to rapidly, accurately and  inexpensively identify the expression profiles of multiple aniogenesis related proteins in several hours. These kits provide a simple array format, and highly sensitive approach to simultaneously detect  up to 43apoptosis related protein expression levels from conditioned media, patient’s sera, cell lysate, tissue lysates and other sources. Comparison of Antibody Array Platforms.

Images: bcl-xL overexpression in ADF glioblastoma and M14 melanoma cells increases CXCL8 expression. Image of membranes from a protein array. Membranes are probed with CM from the (A) glioblastoma control (AN8) and bcl-xL–overexpressing (AXL42 and AXL74) clones and (B) melanoma control (Mneo) and bcl-xL–overexpressing (MXL12 and MXL90) clones. In the negative control (NEG), the CM is replaced with an appropriate mock buffer according to array protocol. The intensity of protein signals for each membrane was compared with the relative positive signals by densitometric analysis. C. Schematic representation of proangiogenic factors that can be detected by the use of the membrane. The CXCL8, TGF-1β, TIMP1, TIMP2, and VEGF protein signals (two spots) are indicated by red, blue, yellow, violet, and green rectangles, respectively, in each image.

Traditionally, cytokines are detected by using ELISA. However, RayBiotech’s approach has several advantages over ELISA. First, and most importantly, our approach can detect many cytokines simultaneously. Secondly, sensitivity is greatly increased. As little as 4 pg/ml of MCP-1 can be detected using the protein array format. In contrast, at least 40 pg/ml of MCP-1 is required to produce a clear signal in an ELISA assay. Furthermore, the detection range is much greater than ELISA. For example, the detection range of IL-2 varies from 25 to 250,000 pg/ml using RayBiotech technology, whereas the detection range varies only within 100-1000 fold in a typical ELISA.

 

NameCatalog #TypeSpeciesApplicationsSizePrice
C-Series Angiogensis Array 1-4AAH-ANG-1-4Membrane-Based ArrayHE20 Angiogenic Factors-4 Sample Kit$489
C-Series Angiogensis Array 1-8AAH-ANG-1-8Membrane-Based ArrayHE20 Angiogenic Factors-8 Sample Kit$729