FLIVO™ Polycaspase Live!, in vivo Apoptosis Kits-NewDesigned for cancer and neurodegenerative disease applications. |
Detecting Tumor Apoptosis
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Are You Killing the Tumor?
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Use FLIVO Polycaspase Live! to monitor the efficacy of chemotherapy.
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If your treatment induces apoptosis in tumor cells, it is probably effective at killing the tumor. Let FLIVO™ guide you to the most effective treatment within hours of administration. more
Detecting Neuron Apoptosis
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Need to Assess Neurodegeneration?
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Use FLIVO Poly Caspase Live! to label dying apoptotic neurons.
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FLIVO crosses the blood-brain barrier and enters neurons. If the neuron is apoptotic and has active caspases, the neuron will fluoresce red or green. more.
Image 1: details: These pictures were taken through a window chamber of 1 live breast cancer tumor growing inside a mouse. Before any treatment was administered, Ploycaspase Live!, green was injected into the mouse to determine how many of the tumor cells were apoptotic: caspase-positive cells turned green, caspase-negative cells were dark. The first picture (A) was taken 45 minutes after Polycaspase Live!, green was injected. Very few of the cells turned green, indicating that most of the tumor is living. The mouse was then injected with a drug (arsenic trioxide, ATO) for 3 hours.Ploycaspase Live!, green was again injected into the mouse, and the second picture (B) was taken 45 minutes later showing the effectiveness of the drug in killing the tumor. Courtesy of Dr. Robert Griffin, University of Minnesota.
Image 2 details: Apoptosis was revealed in subventricular cells of a rat brain after in vivo labeling with Ploycaspase Live!, green. Caspase-positive cells fluoresce bright green. Reagents was injected directly into the ventricle of a live rat brain (gray image, red arrow), the animal was sacrificed and imaged. Courtesy of Peggy Law, U of Toronto.
FLIVO™ Polycaspase Live!-More Customer Data
FLIVO™ Polycaspase Live!-Frequently Asked Questions and Protocol.
More Details on FLIVO Kits
FLIVO™ (FLuorescence in vIVO) probes are non-cytotoxic fluorescent inhibitors of the class of cysteine proteases known as caspases, which are associated with the execution of apoptosis. FAM-FLIVO™ (FAM-VAD-FMK), a green fluorescent probe, is a carboxyfluorescein (FAM) conjugated valylalanylaspartic acid (VAD) fluoromethyl ketone (FMK). The red fluorescent probe, SR-FLIVO™ (SR-VAD-FMK) is a sulforhodamine-B (SR) conjugate of valylalanylaspartic acid (VAD) fluoromethyl ketone (FMK). ICT's FLIVO™ probes preferentially form covalent bonds with active caspases, causing cells undergoing apoptosis to fluoresce. Simple and Most Accurate Method of in vivo Apoptosis Detection | The cell-permeant reagent will diffuse through all cells as it circulates throughout the body. Upon encountering active caspases, FLIVO will form an irreversible covalent bond with a reactive cysteine on the large subunit of the caspase heterodimer, thereby inhibiting further enzymatic activity and labeling its location. The bound FLIVO™ probe will remain inside the cell as long as the cell membrane is intact. Any unbound FLIVO is removed from the circulation of the animal in about an hour. The remaining green or red fluorescent signal in the tissue is a direct measure of caspase activity that occurred at the time the reagent was injected. Eliminate False Positives | FLIVO™ eliminates any false positives that may otherwise arise from post-sacrifice tissue manipulation. This gives a true representation of the induction of apoptosis in vivo as a result of the experimental condition. Fluorescence Analysis Methods |
| Name | Catalog # | Type | Species | Applications | Size | Price |
|---|---|---|---|---|---|---|
| FLIVO™ Polycaspase Live!, green | KF17303 | Kit | 6 Mice 24 Mice | $299 $699 | ||
| FLIVO™ Polycaspase Live!, red | KF17304 | Kit | 6 Mice 24 Mice | $299 $699 |