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Our proprietary in vivo apoptosis detection technology, FLIVO™, is a powerful tool for in vivo apoptosis detection in living animal models. Our fluorescent in vivo apoptosis detection tracers are non-toxic, cell-permeant reagents that specifically label apoptotic cells in vivo for analysis with fluorescence detection methods. The FLIVO tracer technology is based on a common caspase-inhibitor peptide sequence, VAD, and a fluoromethylketone moiety that form covalent bonds to intracellular active caspase enzymes involved in programmed cell death. The FLIVO apoptosis assays have been successfully used in a range of animal models to assess apoptosis as a result of a treatment or biological condition.

Detecting Apoptosis in Tumors-Images: In Vivo Apoptosis Detection: Tumors within living mouse models show high apoptosis levels with NIR-FLIVO 747 after receiving an experimental treatment to induce cell death.
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Detecting Apoptosis in Neurons-Images: Brain abscesses were induced in mice following the intracerebral inoculation of live S. aureus. Animals received intravenous injections of the pan-caspase tracer NIR-FLIVO™ 690 as an apoptosis probe or DyLight®690 (carboxylic acid form) as a control at 17 h post-infection, whereupon signals were acquired 1 h later from brain tissues immediately ex vivo using an IVIS Spectrum (Caliper Life Sciences). The same instrument settings were used to acquire both images. Strong caspase activity, detected with NIR-FLIVO™ 690 (arrows, image on right), was associated with brain abscesses, whereas minimal signal was detected in animals injected with the carboxylic acid control (image on left).

Label, Excitation, and Emission of FLIVO™ Tracers and Controls: 
NIR-FLIVO™ 690 Tracer and Control
: DyLight® 690; 690 nm/ 709 nm 
NIR-FLIVO™ 747 Tracer and Control: DyLight® 747; 747 nm/ 776 nm 
FAM- FLIVO™ Tracer: carboxyfluorescein; 492 nm/ 520 nm
SR-FLIVO™ Tracer: sulforhodamine B; 565 nm/ 600 nm

Note: The NIR-FLIVO® Free Dye Control kits with DyLight® 690 or DyLight® 747 reagents are not "apoptosis control kits", but rather "free dye control kits" for use with the NIR-FLIVO in vivo apoptosis tracers. I am sure you know this, but the way it is currently presented may confuse readers. We don't want any customers to mistake the Free Dye Kits for an apoptosis detection assay. DyLight® is a trademark or Thermo Fisher Scientific, Inc. and its subsidiaries.”

NameCatalog #TypeSpeciesApplicationsSizePrice
NIR FLIVO™ 690 in vivo Apoptosis TracerKF17364KitAllApoptosis DetectionTracer-10 Tests
Full Kit 20 Tests
$229
$429
NIR FLIVO™ 690 in vivo Free Dye Control (DyLight® 690 Carboxylic Acid Form)KF17366KitAllApoptosis DetectionTracer-10 Tests
Full Kit 20 Tests
$229
$289
NIR FLIVO™ 747 in vivo Apoptosis TracerKF17368KitAllApoptosis DetectionTracer-10 Tests
Full Kit 20 Tests
$229
$429
NIR FLIVO™ 747 in vivo Free Dye Control (DyLight® 747 Carboxylic Acid Form)KF17370KitAllApoptosis DetectionTracer-10 Tests
Full Kit 20 Tests
$229
$289
FLIVO™ Polycaspase Live!, greenKF17303KitMApoptosis Detection6 Mice
24 Mice
$299
$699
FLIVO™ Polycaspase Live!, redKF17304KitMApoptosis Detection6 Mice
24 Mice
$299
$699