MitoPT™ Kits

These Kits easily assess changes in mitochondrial membrane potential. Changes in mitochondrial membrane potential can correlate with cytochrome c release and the initiation of apoptosis.

Figure: Using a fluorescence plate reader, healthy cells will give a high OD reading of red fluorescence; apoptotic cells will generate a lower reading of red fluorescence. In this example, Jurkat cells were either treated with DMSO (negative, non-induced cells) or with staurosporine (apoptotic, induced cells) for 4 hours and then labeled with MitoPT™- JC-1 for 15 minutes. As the mitochondrial membrane potential collapsed, indicating apoposis, the amount of red fluorescence dropped by 51% in Jurkat cells from 167 RFU to 82.