Network with Neuromics
Contact Information
Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439
Toll free: 866-350-1500
Int'l phone: 952-374-6161
Fax: 612-677-3976
FLISP™ Serine Protease Detection KitsMeasure chymotrypsin-like protease activation in whole living cells. |
Use our FFCK kit to detect chymotrypsin-like serine proteases that favor phenylalanine;use the FLCK kit for those that favor leucine; use the kits with an additional spacer sequence to reduce steric hindrance at the binding site of the serine protease.
These kits are used with whole living cells and do not requireany cell lysis, fixation, or permeabilization steps.
The chymotrypsin-like serine rotease subfamily plays a key role in a number of diverse physiological processes, including the maintenance of homeostasis, inflammation and immune reactions, apoptosis, and cell survival. For example,chymotrypsin-like serine proteases have been shown to be activated during apoptosis signaling.
Additionally, some chymotrypsin serine protease inhibitors have been shown to induce apoptosis by blocking IkBa degradation, which is important in the NFkB cell survival signaling pathway.
FLISP™ and FLICA™ kits can be used together for dual staining studies.
Images: HL-60 cells were stained with the FLISP Kit containing the FAM-labeled peptide FFCK and FLICA Kit containing SRVAD-FMK after incubation with camptothecin for 3 hours. Cells with active serine proteases stain green (FFCK-FAM) and cells with active caspases stain red (SR-VAD-FMK). Cell displaying either or both activities are easily detected using fluorescence microscopy (left) and FACS (right).
| Name | Catalog # | Type | Species | Applications | Size | Price |
|---|---|---|---|---|---|---|
| FAM-Phe-CMK FLISP™ Assay Kit | KF17314 | Kit | 25 Tests | $164 | ||
| FAM-Phe-CMK FLISP™ Assay Kit | KF17315 | Kit | 100 Tests | $459 | ||
| FAM-Lue-CMK FLISP™ Assay Kit | KF17316 | Kit | 25 Tests | $164 | ||
| FAM-Leu-CMK FLISP™ Assay Kit | KF17317 | Kit | 100 Tests | $459 | ||
| FAM-Spacer-Phe-CMK FLISP™ Assay Kit | KF17322 | Kit | 25 Tests | $164 | ||
| FAM-Spacer-Phe-CMK FLISP™ Assay Kit | KF17323 | Kit | 100 Tests | $459 | ||
| FAM-Spacer-Leu-CMK FLISP™ Assay Kit | KF17324 | Kit | 25 Tests | $164 | ||
| FAM-Spacer-Leu-CMK FLISP™ Assay Kit | KF17323 | Kit | 100 Tests | $459 | ||
| SR-101-Phe-CMK FLISP™ Assay Kit | KF17318 | Kit | 25 Tests | $164 | ||
| SR-101-Phe-CMK FLISP™ Assay Kit | KF17319 | Kit | 100 Tests | $459 | ||
| SR-101-Leu-CMK FLISP™ Assay Kit | KF17321 | Kit | 100 Tests | $459 |