Cytotoxicity Test Kits

Cytolytic activity is an important process by which the immune system eliminates compromised cells in the body. Whether describing antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity, the activation of the immune system leads to the removal of target cells infected by pathogens or transformed by cancer. Alternatively, cytotoxicity can simply refer to any compound (drug or toxin) that also results in killing of the target cells.

Figure:  Based on flow cytometric data, the formula [R2/(R2+R1)] X 100 was used to calculate cytotoxicity. NK-killed necrotic cells are shown in the maroon line, and apoptotic cells are shown as the blue line. When added together, the total level of cell death is calculated as the black line. In this example, cytotoxicity increased as more NK effector cells were combined with target cells. As the ratio of Effector:Target-cells increased from 12.5:1 to 100:1 the percentage of target cells dying increased from 11% to 60%.

 We offer two easy, reliable, and accurate assays to measure cytotoxicity. The Basic Cytotoxicity Test simplifies measurement of necrotic and membrane-compromised cells. The Total Cytotoxicity Test  further distinguishes apoptosis from necrosis, leading to a more accurate assessment of cytolytic activity. At least 10% more cytolytic activity is often measured when apoptotic cells are also identified. ICT's kits provide a direct measurement of cytotoxicity rather than using an indirect indicator, such as the release of LDH or ATP enzymes, and there is no need to lyse the cells. These assays do not use any radioisotopes, they are much safer and more cost effective to run than 51Cr assays. Highlights:

• Easy - run samples in a single tube
• Safe – no radioactivity
•  Fast - finish in one day Sensitive - measure killed and apoptotic cells
• Accurate – analyze cells with a flow cytometer

Cytotoxicity Test Kits FAQs