ELISA BuffersCoating, blocking and wash buffers |
All blocking buffers contain proprietary additives that stabilize the antigenic and functional regions of the coated protein by maintaining the water of hydration during the drying and storage process. These buffers also contain inert additives that block any "sticky" nonspecific binding regions of the adsorbed protein, and any uncoated regions of the polystyrene plate surface. This reduces nonspecific binding of the conjugate and random deposits of sample proteins, thus reducing background noise and increasing the specific signal.
Buffers also include an antimicrobial agent to prevent bacterial growth during the blocking process (which allows blocking to be done at room temperature) and to extend the useful shelf-life of the plates.
Energize your ELISA Assays with our Buffers:
- By saving valuable reagents.
- By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when this buffer is used.
- By increasing the shelf-life of your plates. Depending on the activity of the coated protein, plates may be stored at RT or 2°-8°C for several months, or even years under proper conditions.
- By increasing reproducibility.
- By using consistent reagents from a reliable source, your ELISAs may become more reproducible.
- By increasing the signal of the ELISA. If the antibody or antigen is not sticking to the plate, or unwanted proteins are interfering with binding, the plate will have an overall low specific signal. CB1 may foster adsorption and reduce interference.
- By decreasing background noise. If excess proteins are sticking to the plate, plate will have overall high backgrounds (a high signal overall, but a low specific signal). CB1 may reduce interference.
| Name | Catalog # | Type | Species | Applications | Size | Price |
|---|---|---|---|---|---|---|
| Plate Coating Buffer-CB1 | KF17336-1 | Coating Buffer | E | 100 ml 500 ml 1 L 10 L | $36 $109 $219 $1,899 | |
| General Blocking Buffer-BB1 | KF17337-1 | Blocking Buffer | E | 100 ml 500 ml 1 L 10 L | $36 $109 $219 $1,899 | |
| Neptune Blocking Buffer-BB2 | KF17338-1 | Blocking Buffer | E | 100 ml 500 ml 1 L 10 L | $44 $149 $259 $2,349 | |
| SynBlock Blocking Buffer-BB3 | KF17339-1 | Blocking Buffer | E | 100 ml 500 ml 1 L 10 L | $44 $149 $259 $2,349 | |
| 3 Blocking Buffer Sample Pack | KF17340 | Blocking Buffer | E | 100 ml | $105 | |
| Wash Buffer | KF17356-1 | Wash Buffer | E | 100 ml 500 ml 1 L 10 L | $35 $99 $199 $1,789 |