ELISA Assay DiluentsEqualize matrix sample vs standard curve differences |
Reduce matrix effects with assay diluents
These assay diluents are proprietary buffer formulations designed to equalize any differences between the sample matrix (serum, plasma, urine, cell culture fluid) and the diluent used to generate the standard curve. These diluents also reduce nonspecific interactions between the sample matrix proteins and the plate surface which translates into lower background noise.
Assay diluents are pipetted directly onto the plate just prior to adding the samples, and may be used with neat or diluted samples, depending on the characteristics of the sample matrix and the target range of the assay. Since different sample matrices have unique characteristics when run in an ELISA, they must be matched with specific assay diluents. Use of an assay diluent reduces the effects of the sample matrix and variation among samples, without pre-dilution of the samples.
We have 4 proprietary assay diluent formulations for use with sandwich and antigen-down ELISAs:
| Name | Catalog # | Type | Species | Applications | Size | Price |
|---|---|---|---|---|---|---|
| General Assay Diluent-AD1 | KF17347-1 | 100 ml 500 ml 1 L 10 L | $45 $139 $240 $2,160 | |||
| IgM Reducing Assay Diluent-AD2 | KF17378-1 | 100 ml 500 ml 1 L 10 L | $49 $149 $261 $2,349 | |||
| Neptune Assay Diluent-AD3 | KF17349-1 | 100 ml 500 ml 1 L 10 L | $49 $149 $261 $2,349 | |||
| Antigen-Down Assay Diluent-AD4 | KF17350-1 | 100 ml 500 ml 1 L 10 L | $58 $178 $324 $2,916 | |||
| All Assay Diluents Sample Pack | KF17351-1 | Assay Diluent | E | 4X100 ml | $170 |