ChromaLink™ Biotin Labeling Kits

ChromaLink^TM One-Shot Antibody Labeling Kit offers the complete solution to the quantitative biotinylation of 100 ug of antibody . The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation. A simple Å280 and Å354 measurement determines the number of biotins per antibody molecule.

The labeling process used in the One-Shot kit is based on a patented UV-traceable linker, Sulfo-ChromaLink^TM Biotin (see Figure).  This advanced protein biotinylation reagent contains an aromatic water-soluble N-hydroxy-sulfosuccinimidyl ester functional group (a), which efficiently modifies protein lysine residues in an aqueous phosphate buffer system.  The linker also possesses an embedded bis-aryl hydrazone structure (b), which forms the compound’s UV-traceable chromophore.  This traceable signature enables the non-destructive and sensitive quantification of biotin (c), attached to the antibody.  All these diverse chemical elements are linked together through a long-chain PEG3 spacer (d), which preserves streptavidin/biotin affinity while simultaneously enhancing antibody solubility.

Figure: Structure of hBiotin (C₃₈H₄₉N₈NaO₁₃S₂) (M.W. 912.96)