Strep-Well Procedure

The procedure uses a 96-well filter plate to clear bacterial lysates by vacuum filtration.

1. The cultures are chemically lysed.
   
2. The lysates are cleared by vacuum filtration through the 96-well filter plate.
   
3. The cleared protein lysates are applied to the Strep-Well HT Purification Plate.
   
4. After washing, the purified proteins are eluted in 300-450 µl elution buffer.

To prevent cross-contamination of the samples a 96-well wash plate is available.
The eluates are collected in a 96-well receiver plate.
Washing and elution steps are carried out on a vacuum manifold. Ready-to-run protocols are provided.