Network with Neuromics
Contact Information
Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439
Toll free: 866-350-1500
Int'l phone: 952-374-6161
Fax: 612-677-3976
Strep-Tactin Column-Resin Comparison Table
2011-08-29| Strep-Tactin Sepharose | Strep-Tactin Superflow | Strep-Tactin Superflow high capacity | Strep-Tactin MacroPrep | Strep-Tactin POROS 20 | Strep-Tactin POROS 50 | |
| Gravity flow column | Yes | Yes | Yes | Yes | No | No |
| Batch purification | For One- Strep-tag only | For One- Strep-tag only | For One- Strep-tag only | For One- Strep-tag only | For One- Strep-tag only | For One- Strep-tag only |
| FPLC | No | Yes | Yes | Yes | Yes | Yes |
| HPLC | No | Yes | Yes | Yes | Yes | Yes |
| Binding capacity | 50 - 100 nmol/ml | 50 - 100 nmol/ml | 150 - 500 nmol/ml | 50 - 100 nmol/ml | 25 - 50 nmol/ml | 25 - 50 nmol/ml |
| Support | Sepharose 4FF | Superflow 6 | Superflow 6 | MacroPrep 50 | POROS 20 | POROS 50 |
| Bead structure | agarose | 6% agarose, crosslinked | 6% agarose, crosslinked | polymethacrylate | polystyrene | polystyrene |
| Bead size | 45 - 165 µm | 60 - 160 µm spherical | 60 - 160 µm spherical | 50 µm | 20 µm | 50 µm |
| Exclusion limit | 3 x 107 | 6 x 106 | 6 x 106 | 1 x 106 | n.d. | n.d. |
| Recommended linear flow rate* | up to 30 cm/h | up to 300 cm/h | up to 300 cm/h | up to 300 cm/h | 300 - 500 cm/h | 300 - 500 cm/h |
| pH stability | 4 - 9 | 2 - 13 | 2 - 13 | 2 - 12 | 1 - 13 | 1 - 13 |
| Extreme pH as listed above may be used for short cleaning procedures but should be changed to pH 8.0 for storage and Strep-tag protein binding | ||||||
| Max. pressure | Gravity flow | n.d | n.d | n.d | 170 bar | 170 bar |
| Form | buffered 50% suspension | buffered 50% suspension | buffered 50% suspension | buffered 50% suspension | buffered 50% suspension | buffered 50% suspension |
| Storage | 4 °C, do not freeze | 4 °C, do not freeze | 4 °C, do not freeze | 4 °C, do not freeze | 4 °C, do not freeze | 4 °C, do not freeze |
| Shipment | RT | RT | RT | RT | RT | RT |
| ** For Strep-tagII only recommended if expression rate >1mg/ml otherwise use One-Strep-tag | ||||||