StarGate® offers rapid and highly efficient subcloning of an arbitrary gene – initially cloned into a Donor Vector - to simultaneously fuse it with many different genetic surroundings via transfer into Acceptor Vectors to generate Destination Vectors. The latter enable the efficient expression of your protein with various features (e.g. different tags and different promoters) in different hosts.

 Features include:

  • Minimal modification of the gene of interest due to short combinatorial sites(4 bases only).
  • Easy-to-handle subcloning procedure.
  • Systematic screening of different elements (e.g. tags/promoters) in a variety of hosts.
  • Inherent high level cloning efficiency due to a directed reaction(no equilibrium.
  • Availability of a multitude of combinatorial sites for combinatorial cloning.