HALO®-96 Tox and HALO®-384 HT

Designed for toxicity studies in Human/Animal Lympho-Hematopoietic Stem and Progenitor Cell Assays
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A Highly Predictive, In Vitro Stem and Progenitor Cell Hemotoxicity Screening and Testing Platform for all Stages of Drug Development and Xenobiotics.

HALO®-96 Tox and HALO®-384 HT Hemotoxicity Screening Kits are designed to so that toxicity/apoptosis can be determined at the earliest stages of blood development. This is where the majority of compounds act. In addtion they can be used for all stages of drug development, including patient monitoring during clinical trials.

Diagram: Measuring drug toxxicity in hematopoietic stem cells. Shows different types of dose responses and ranked according to IC50 values.

Kits applications:
  • Predictive in vitro to in vivo surrogate assay for human hemotoxicity.
  • Predictive in vitro to in vivo surrogate assay for all animal hemotoxicity testing.
  • Predictive in vitro stem and progenitor hemotoxicity screening and testing platform for all stages of drug development, including patient monitoring during clinical trials.
  • Predictive in vitro stem and progenitor hemotoxicity screening and testing for xenobiotic agents. Incorporation into routine high throughput ADME/Tox screening.
  • Drug / compound ranking according to species and/or cell population sensitivity.

Cell Populations, Tissue, Species Detected using HALO®-Tox HT

Stem Cells
  • HPP-SP 1: IL-3, IL-6, SCF, TPO, Flt3-L
  • HPP-SP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L
  • CFC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
  • CFC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
  • CFC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
  • CFC-GEM 2: EPO, GM-CSF, IL-3, SCF
  • CFC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF
Progenitor Cells
  • BFU-E 1: EPO, IL-3, SCF
  •  BFU-E 2: EPO
  • GM-CFC 1: GM-CSF, IL-3, SCF
  • GM-CFC 2: GM-CSF, G-CSF, IL-3, SCF
  • GM-CFC 3: GM-CSF
  • Mk-CFC 1: TPO, IL-3, SCF Mk-CFC 2: TPO
Lymphopoietic cells
  • T-CFC: IL-2
  • B-CFC: IL-7
Species
Human,Non-human primate, Dog, Rat, Mouse Horse, Pig and Sheep.

Tissues:  Bone marrow: human and animal tissues; Peripheral blood: human and animal tissues Umbilical cord blood:  human; Spleen: rodents Fetal and adult liver;  Yolk sac: rodents

Note: No growth factors: Add growth factors/cytokines of choice.

Biolumonics Basic Procedures(pdf - 323Kb)
Fast and easy to learn
NameCatalog #TypeSpeciesApplicationsSizePrice
HALO-96® Tox-HumanK2-0-2HHKitHCell Assays1X96 Well Plate$1,405
HALO-96® Tox-DogK2-0-2CHKitDCell Assays1X96 Well Plate$1,405
HALO-96® Tox-MouseK2-0-2MHKitMCell Assays1X96 Well Plate$1,405
HALO-96® Tox-PrimateK2-0-2PrHKitPrCell Assays1X96 Well Plate$1,405
HALO-96® Tox-RatK2-0-2RHKitRCell Assays1X96 Well Plate$1,405
HALO-384® Tox-HumanK3-0-2HHKitHCell Assays2X384 Well Plates$1,405
HALO-384® Tox-DogK3-0-2CHKitDCell Assays2X384 Well Plates$1,405
HALO-384® Tox-MouseK3-0-2MHKitMCell Assays2X384 Well Plates$1,405
HALO-384® Tox-PrimateK3-0-2PrHKitPrCell Assays2X384 Well Plates$1,405
HALO-384® Tox-RatK3-0-2RHKitRCell Assays3X384 Well Plates$1,405