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The Streptamers® for T cell expansion are novel reagents for polyclonal expansion of T cells: 
  • They are non-magnetic soluble protein complexes generated by multimerization of αCD3- and αCD28 Fab-Streps with a Strep-Tactin® multimer.
  • They are completely reversible reagents, i.e. they can be removed from the cells by the addition of biotin.
  • The Streptamer® CD3/CD28 premix for T cell expansion is easy and fast with ready-to-use reagents.
  • FACS Data available for the expansion of T-Cells using CD4+, CD8+, CD25 and CD 65 markers.
Image: Human T cells were isolated with the CD3 Fab Streptamer® Isolation Kit MB (6-8000-201), stained with CFSE and cultured in a 48 well plate for 5 days. Proliferation was measured in pregated CD4+ (upper panel) or CD8+ T cells (lower panel) using Flow Cytometry. Dead cells were excluded from the analysis using PI.
 
The premixed (1X480 ul.) and unmixed kits (1X160 ul.) contain the following reagents:

Components

CD3 Fab-Strep for cell expansion (#6-8910-016)
CD28 Fab-Strep for cell expansion (#6-8911-016)
Strep-Tactin® multimer for cell expansion (#6-8912-016)

Amounts

1 x 160 µl
1 x 160 µl
1 x 160 µl

NameCatalog #TypeSpeciesApplicationsSizePrice
Streptamer® CD3/CD28 Premixed6-8901-000T-Cell ExpansionHCell Assays1X480 ul$225
Streptamer® CD3/CD28 Unmixed6-8900-000T-Cell ExpansionHCell Assays3X160 ul$245
D-Biotin2-1016-002T-Cell Expansion2 g
5 g
$107
$179
Biotin Blocking Buffer2-0501-002Blocking BufferNot ApplicableWB2 ml$99