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PC351011 Hippocampal pair$295.00Buy Now | Add to Cart
 
Type: Primary Cells
 
Applications: Cell Assays
E=ELISA; FC=Flow Cytometry; ICC=Immunocytochemistry; IF=Immunofluorescence; IP=Immunoprecipitation; IHC=Immunohistochemistry; SE=Sandwich ELISA; WB=Western blotting; NB=Neutralization of Bioactivity; FACS; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; BSM=Biosactive Small Molecule or Peptide; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; AC=Adherent Cell Assays; NAC=Non-adherent Cell Assays; CDM=Cell Differentiation Media; BSC-CM5= Biacore Sensor Chip CM5; FM=Fluorescent Micsroscopy; ; ; ; ; ; ; ; ; ;
Species Reactivity: R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Description/Data:
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Primary Rat Hippocampal Cells are live neurons isolated from micro surgically dissected regions of day 18 embryonic Sprague/Dawley rat brain. These cells are prepared fresh each week and shipped in a nutrient rich medium that keeps the cells alive for up to 7 days under refrigeration. For longer term culturing protocol (120 days) see: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0058996

Image: E18 hippocampal neurons stained with Tau (red) and Doublecortin (green). The two proteins overlap in the proximal dendrites (yellow) Axons (low doublecortin content) are red. Blue staining is the nuclear DNA. 

Hippocampal Neurons: Customer Publications

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Data and FAQs

Companion Products:

NbActiv1 - Neuron growth media.

Poly-D-Lysine Coated Coverslips-2 Glass coverslips, (1.76cm2) coated with poly-D-lysine, sterile, in PBS, shelf-life 1 week, per pair.

Please note we also provide Adult Neurons in Qtys of 4 (or more) for $995.  To order call brett @ 866-350-1500 or e-mail: brett@neuromics.com

Picture

Images: A–D: Uptake of fluorescein labeled Aβ40 (F-Aβ40) and Alexa Fluor® 633 labeled transferrin (AF633-Trf), clathrin-mediated endocytosis marker, in rat primary hippocampal (RPH) neurons following 30 min incubation at 37°C. (A) F-Aβ40 uptake; (B) Uptake of AF633-Trf; (C) Superimposition of images A and B; (D) Overlay of fluorescence images on the DIC image of RPH neurons. E–G: Uptake of F-Aβ40 and AF633-Trf in RPH neurons at 4°C. (E) Uptake of F-Aβ40; (F) No significant neuronal uptake of AF633-Trf at 4°C; (G) Superimposition of images D and E on the DIC image of RPH neurons; H–J: Uptake of F-Aβ40 and AF633-Trf in RPH neurons treated with 10 mM Sodium Azide and 50 mM 2-deoxy glucose (cellular ATP depletion agents). (H) Uptake of F-Aβ40; (I) No significant cellular uptake of AF633-Trf was observed; (J) Superimposition of images H & I on the DIC image of neurons. doi:10.1371/journal.pone.0004627.g010