Picture

Image: hN2 Human Neurons Discovery Kit-IF Image of differentiated in the presence of 25 ng/ml GDNF. Cells were also counterstained for nuclei (DAPI) and  Tuj 1-Neuron-specific class III beta-tubulin and TH-Tyrosine Hydroxylase. Dopaminergic Neural Differentiation: Human neural progenitor cells were grown on polyornithine/laminin coated 35mm plates (Falcon) for flow cytometry or on polyornithine/laminin coated 4 well slides (Falcon) for immunocytochemistry staining. Cells were plated in proliferation medium. At 24 hours post-plating, the medium was changed to neural differentiation medium (proliferation medium without bFGF) plus or minus 25 ng/ml GDNF (Neuromics). Medium were changed every three days. Cells were harvested at differentiation day 0, 3, 7, 14 and 21 for further analysis.

For related reagents, visit our Stem Cell Reagents category or our extensive listing of Axon Growth and Guidance,  Neurotrophins and Growth Factors or Neuron/Glial Markers Antibodies

NameCatalog #TypeSpeciesApplicationsSizePrice
Activin APR15010ProteinH; M; R10 ug
50 ug
$250
$760
Activin A, CFPR15010CFProteinH; M; R10 ug
50 ug
$250
$760
Activin ABPR15011ProteinH5 ug$330
Activin AB, CFPR15011CFProteinH5 ug$330
Activin BPR15012ProteinH5 ug
25 ug
$250
$760
Activin B, CFPR15012CFProteinH5 ug
25 ug
$250
$760
BDNFPR29001ProteinH; M2 ug
10 ug
100 ug
1 mg
$139
$339
$1,112
$7,275
BDNFPR27080-2ProteinH2 ug
10 ug
$108
$205
BDNFPR15020ProteinH5 ug
25 ug
$220
$660
BDNFPR27080-2ProteinH2 ug
10 ug
$108
$205
BDNF, CFPR15020CFProteinH5 ug
25 ug
$220
$660
BMP-2PR27023-2ProteinH2 ug
10 ug
$108
$205
BMP-2PR15013ProteinH10 ug$450
BMP-2 (E. coli-expressed), CFPR15014CFProteinH10 ug$200
BMP-2 (E.coli-expressed)PR15014ProteinH10 ug$200
BMP-2, CFPR15013CFProteinH10 ug$450
BMP-4PR27024-2ProteinH2 ug
10 ug
$108
$205
BMP-4PR15015ProteinH10 ug$435
BMP-4, CFPR15015CFProteinH10 ug$435
BMP-7PR27026-2ProteinH2 ug
10 ug
$108
$205
BMP-7 CHOPR27001-2ProteinH2 ug
10 ug
$108
$205
CD105 (Endoglin)PR27035-2ProteinH2 ug
5 ug
$225
$350
CD14PR27030-10ProteinH10 ug
50 ug
$225
$350
CD14PR27031-2ProteinM2 ug
5 ug
$225
$350
CD146PR27039-2ProteinH2 ug
5 ug
$225
$350
CD4 (26-226)PR27038-2ProteinH2 ug
5 ug
$225
$350
CD4 (Soluble)PR27032-2ProteinH2 ug
10 ug
$108
$205
CD40 Ligand/TRAPPR29002-10ProteinH10 ug
50 ug
1 mg
$139
$338
$2,794
CD40 Ligand/TRAP (Soluble)PR27033-10ProteinH10 ug
50 ug
$225
$350
CNTFPR27005-5ProteinH5 ug
20 ug
$108
$205
EG-VEGFPR29003-5ProteinH5 ug
20 ug
100 ug
1 mg
$139
$339
$870
$4,219
EGFPR27006-100ProteinH100 ug
500 ug
$108
$205
EGFPR27009-100ProteinM100 ug
500 ug
$108
$205
EGF PichiaPR27007-100ProteinH100 ug
500 ug
$108
$205
EGF, carrier-freePR15005CFProteinH200 ug
1 mg
$120
$400
EPO-alpha FC HumanPR27064-2ProteinH2 ug
10 ug
$108
$205
EPO-alpha HumanPR27065-5ProteinH5 ug
50 ug
$225
$350
EphA4/Fc Chimera, CFPR15069CF-200ProteinM200 ug$265
FGF (154), basicPR29000-10ProteinH10 ug
50 ug
1 mg
$136
$332
$1,815
FGF basic (146 aa)PR15002ProteinH25 ug$175
FGF basic (146 aa), carrier-freePR15002CFProteinH25 ug$175
FGF basic (157 aa)PR15003ProteinM25 ug$245
FGF basic (157 aa), carrier-freePR15003CFProteinM25 ug$245
FGF-1, acidicPR27011-10ProteinH10 ug
50 ug
$108
$205
FGF-1, acidic, Sf9PR27012-2ProteinH2 ug
10 ug
$108
$205
FGF-19PR27019-5ProteinH5 ug
25 ug
$108
$205
FGF-2PR27016-2ProteinM2 ug
10 ug
$108
$205
FGF-2 (Bovine) basicPR27015-10ProteinB10 ug
50 ug
$108
$205
FGF-2, basicPR27013-10ProteinH10 ug
50 ug
$108
$205
FGF-2, basic, Sf9PR27014-2ProteinH2 ug
10 ug
$108
$205
FGF-22PR27021-2ProteinH2 ug
10 ug
$108
$205
FGF-8bPR15004ProteinM25 ug$245
FGF-8b, carrier-freePR15004CFProteinM25 ug$245
FGF-8cPR15017ProteinM25 ug$250
FGF-8c, carrier-freePR15017CFProteinM25 ug$250
FGF-9PR27017-2ProteinH2 ug
20 ug
$108
$205
FGF-9, mousePR27018-2ProteinM2 ug
10 ug
$108
$205
Fibronectin, carrier-freePR15001CFProteinBAdherent Cell Assays1 mg
5 mg
$125
$315
G - CSF HumanPR27066-2ProteinH2 ug
10 ug
$225
$350
G - CSF MousePR27067-2ProteinM2 ug
10 ug
$108
$205
GDF-5PR27028-10ProteinH10 ug
50 ug
$108
$205
GDNFPR29004-2ProteinH2 ug
10 ug
100 ug
1 mg
$165
$339
$1,112
$6,350
GDNF (Human, Mouse)PR27022-2ProteinH; M2 ug
10 ug
$108
$205
GDNF (Rat)PR15008ProteinR10 ug
50 ug
$285
$940
GDNF (Rat), Carrier FreePR15008CFProteinR10 ug
50 ug
$285
$940
GM - CSF HumanPR27068-2ProteinH2 ug
10 ug
$108
$205
GM - CSF Human, PichiaPR27070-2ProteinH2 ug
10 ug
$108
$205
GM - CSF MousePR27074-2ProteinM2 ug
10 ug
$108
$205
GM-CSF+ IL-3 (PIXY321) Fusion ProteinPR27073-2ProteinH2 ug
10 ug
$108
$205
HGF CHOPR27049-2ProteinH2ug
10 ug
$108
$205
HGF HumanPR27050-2ProteinH2 ug
10 ug
$108
$205
Heregulin-Beta2PR27046-10ProteinH10 ug
50 ug
$225
$350
ICAM-1/CD54PR27037-2ProteinH2 ug
10 ug
$108
$205
IGF-I, CFPR15022CFProteinH50 ug
250 ug
$170
$545
IGF-I, CFPR15023CFProteinM50 ug$175
IGF-IIPR15045CFProteinM50 ug$175
IL-1BetaPR15071-10ProteinR10 ug
50 ug
$285
$840
IL-3 (Human)PR27075-2ProteinM2 ug
10 ug
$108
$205
IL-3 (Mouse)PR27076-2ProteinM2 ug
10 ug
$108
$205
IL-3 (Rat)PR27077-2ProteinR2 ug
10 ug
$108
$205
ISOKine-EGFPR80002-100ProteinHCell Assays100 ug$225
ISOKine-Flt3-Ligand, human-NEWPR80004-10ProteinHCell Assays10 ug-10(6) u
100 ug-10(7) u
1 mg-10(8) u
$129
$529
$2,649
ISOKine-bFGFPR80001-10ProteinH; MCell Assays10 ug
50 ug
100 ug
$65
$169
$225
ISOKine-Leukemia Inhibitory Factor (LIF), human-NEWPR80003-10ProteinHCell Assays10 ug-10(6) u
100 ug-10(7) u
1 mg-10(8) u
$90
$395
$2,475
ISOKine-Leukemia Inhibitory Factor (LIF), mouse-NEWPR80000-10ProteinMCell Assays10 ug-10(6) u
50 ug-5X10(6) u
100 ug-10(7) u
1 mg-10(8) u
$90
$250
$395
$2,475
ISOKine-SCF, human-NEWPR80005-10ProteinHCell Assays10 ug-10(6) u
100 ug-10(7) u
1 mg-10(8) u
$90
$395
$2,475
Indian Hedgehog (C28II) N-Term Peptide, CFPR15021CFProteinM25 ug$315
Indian Hedgehog (C28II) N-Terminal PeptidePR15021ProteinM25 ug$315
Leukemia Inhibitory Factor (LIF), humanPR16101ProteinH5 ug
10 ug
$295
$435
Leukemia Inhibitory Factor (LIF), mousePR16102ProteinM5 ug
10 ug
$295
$435
Leukemia Inhibitory Factor (LIF), ratPR16103ProteinR5 ug
10 ug
$295
$435
MIF HumanPR27042-5ProteinH5 ug
25 ug
$108
$205
NGF-b, Human, CHOPR27002-5ProteinH5 ug
20 ug
$108
$205
NGF-b, MousePR27003-5ProteinM5 ug
20 ug
$108
$205
NogginPR27117-5ProteinH5 ug
20 ug
$108
$205
Noggin/Fc Chimera, Carrier FreePR15070CF-50ProteinH50 ug$315
OMgpPR15054-25ProteinH25 ug$315
PD-ECGFPR15044ProteinH10 ug$335
PDGF - A HumanPR27059-5ProteinH5 ug
20 ug
$108
$205
PDGF - AA HumanPR27061-2ProteinH2 ug
10 ug
$108
$205
PDGF - AB HumanPR27062-2ProteinH2 ug
10 ug
$108
$205
PDGF - B HumanPR27060-5ProteinH5 ug
20 ug
$108
$205
PDGF-BBPR15027ProteinR50 ug$265
PDGF-BB, CFPR15026CFProteinH10 ug
50 ug
$250
$795
PDGF-BB, CFPR15027CFProteinR50 ug$265
PGP9.5PR27120-5ProteinH5 ug
25 ug
$108
$205
ProNGFPR27004-2ProteinH2 ug
10 ug
$108
$205
RetPR15043-50ProteinH50 ug$315
Ret, CFPR15043CF-10ProteinH10 ug$315
SMAD2PR27147-2ProteinH2 ug
10 ug
$108
$205
SMAD4PR27148-10ProteinH10 ug
50 ug
$108
$205
Sonic HedgehogPR15000ProteinM25 ug
25 ug
$255
$255
Sonic Hedgehog (C24II) N-TerminusPR15018ProteinH25 ug$315
Sonic Hedgehog (C24II) N-Terminus, CFPR15018CFProteinH25 ug$315
Sonic Hedgehog N-Terminus (Shh-N)PR15019ProteinH25 ug$285
Sonic Hedgehog N-Terminus (Shh-N), CFPR15019CFProteinH25 ug$285
Stem Cell Factor/KIT LigandPR27121-2ProteinR2 ug
20 ug
$108
$205
Stem Cell Factor/KIT LigandPR27122-2ProteinH2 ug
10 ug
$108
$205
TGF Beta1PR15009ProteinH1 ug$250
TGF-alpha, CFPR15016CFProteinH100 ug
500 ug
$315
$990
TGFBIPR27154-10ProteinH10 ug
50 ug
$108
$205
TIE-1 Fc ChimeraPR27153-2ProteinH2 ug
10 ug
$108
$205
TIE-2/CD202b Fc ChimeraPR27152-2ProteinH2 ug
10 ug
$108
$205
Transforming Growth Factors (TGFs)
TrkA/Chimera, Carrier FreePR15051CF-50ProteinR50 ug$325
TrkA/Chimera, Carrier FreePR15050CF-50ProteinH50 ug$325
VEGFPR15028ProteinD10 ug$315
VEGF 120PR15033ProteinM25 ug
5 ug
$825
$255
VEGF 121PR15029ProteinH25 ug
5 ug
$905
$280
VEGF 121, CFPR15029CFProteinH5 ug
25 ug
$280
$905
VEGF 162PR15030ProteinH25 ug$315
VEGF 162, CFPR15030CFProteinH25 ug$315
VEGF 164PR15034ProteinM25 ug
5 ug
$825
$255
VEGF 164PR15035ProteinR10 ug
50 ug
$315
$905
VEGF 164, CFPR15034CFProteinM5 ug
25 ug
$255
$825
VEGF 164, CFPR15035CFProteinR50 ug
10 ug
$905
$315
VEGF 165PR15031ProteinH10 ug
50 ug
$280
$905
VEGF 165, CFPR15031CFProteinH50 ug
10 ug
$905
$280
VEGF, CFPR15028CFProtein10 ug$315
VEGF-CPR27170ProteinH2 ug
10 ug
1 mg
$108
$195
$2,799
VEGF/PIGF HeterodimerPR15032ProteinH5 ug$315
VEGF/PIGF Heterodimer, CFPR15032CFProteinH5 ug$315
Visfatin (PBEF), His TaggedPR27140-5ProteinH5 ug
25 ug
$108
$205
WIF1PR27150-2ProteinH2 ug
5 ug
$195
$290
Human Wnt3aPR31000-1ProteinH1 ug
2 ug
10 ug
$99
$139
$399
Mouse Wnt3aPR31001-1ProteinM1 ug
2 ug
10 ug
$119
$199
$699