Collagen IV

Human ECM Protein
Datasheet and Coating Protocol(pdf - 80Kb)
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Type: ECM
 
Applications: 3-D Cell Cultures
ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; WB=Western blotting; FC=Flow Cytometry; IP=Immunoprecipitation; E=ELISA; NB=Neutralization of Bioactivity; FACS; FM=Fluorescent Micsroscopy; ; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; ; ; AC=Adherent Cell Assays; ; ; NAC=Non-adherent Cell Assays; ; ; BSC-CM5= Biacore Sensor Chip CM5; ;
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins
Format: liquid
 
Description/Data:

Collagen can be divided into several families according to the types of structure they form. Collagen Type IV is one of the most abundant proteins in the extracellular matrix (ECM). Basement membranes are thin, specialized ECMs that surround most tissues

Collagen Type IV is a heterotrimeric molecule containing two α1-like and one α2-like chain. Vertebrates have three α1 and three α2-like genes. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types.

Collagen Type IV is produced by human fibroblasts and epithelial cells in a co-culture system, which produces an ECM in vitro. The Collagen Type IV is then purified biochemically and provided sterile.