Catalog NumberSizePrice (USD)Shopping Cart
HN600024,000,000 Cells$1,395.00   $1,395.00Buy Now | Add to Cart
 
Type: Primary Cells
 
Applications: Cell Assays
E=ELISA; FC=Flow Cytometry; ICC=Immunocytochemistry; IF=Immunofluorescence; IP=Immunoprecipitation; IHC=Immunohistochemistry; SE=Sandwich ELISA; WB=Western blotting; NB=Neutralization of Bioactivity; FACS; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; BSM=Biosactive Small Molecule or Peptide; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; AC=Adherent Cell Assays; NAC=Non-adherent Cell Assays; CDM=Cell Differentiation Media; BSC-CM5= Biacore Sensor Chip CM5; FM=Fluorescent Micsroscopy; ; ; ; ; ; ; ; ; ;
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Frozen
 
Description/Data:
Picture

hN2™ Screening Kit contains the differentiated neuronal cells and optimized medium plus supplement for thawing and maintaining the cells in culture and is an excellent choice for high-throughput (96- and 384-well) formatted assays.

Applications: Gene Expression Analysis, Western blotting, Flow Cytometry, Immunocytochemistry, FACS sorting, DNA Microarray, RT-PCR, Neurite Outgrowth assays, FLIPR calcium assays, cytotoxicity, and second messenger signaling. 

images: Confocal image of STEMEZ™ hN2™ cells stained for the neuronal markers β III tubulin (green) and MAP2 (red) and for nuclei (blue).

Kit Contents:

Component:

  • 4 vial cells
  • 500 mL AB2TM Basal Medium
  • ANSTM Supplement

Storage Conditions

  • Liquid nitrogen
  • 2-8 ºC protected from light
  • <-20 ºC; if it thaws, store at 2-8 ºC; do NOT refreeze

Please note: There is enough media included to keep the cells viable for up to 1 month.

Required but Not Included:

  • 1 mL 100x Pen-Strep (Optional)
  • Coated plates or dishes with Matrigel® from BD
  • L-Glutamine (200 mM)-Invitrogen-25030081
  • LIF-Millipore-LIF1010
  • Dulbecco's Modified Eagle's Medium

DNA fingerprint cells:    The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) HESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.

Viral tests cells:  This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative. (Tests performed by GIVF Laboratories).

Delivery Time: 7-10 days

Picture[2]

hN2 cells can produce inward currents that generate action potentials.

Figure: Isolated hN2 with significant neurite growth 1 week after plating . This cell was subjected to whole cell voltage clamp utilizing a potassium gluconate based intracellular solution. (B) Voltage gated inward and outward currents were elicited from this cell with depolarizing voltage steps. (C) Inward currents from another cell (potassium gluconate intracellular) were abolished by local application of 1 µM tetrodotoxin (red trace) while outward currents remained. Inward current recovered as TTX washed out of the region (green trace). (D) A different cell which exhibited voltage activated inward currents that inactivated in response to a 50 ms prepulse at different membrane potentials. The experiment was done 27 days after the removal of bFGF. A cesium gluconate based intracellular solution was used for this experiment to block outward potassium currents. The membrane potential for half maximal inactivation by standard Boltzman fitting (red line) was -40.1 mV with a slope of 4.7. (E) Recovery from fast inactivation utilizing a paired pulse protocol in the same cell as C. The single exponential time constant for recovery of inactivation was 1.7 ms (red line). (F) A different cell which elicited an overshooting action potential upon current injection under whole cell current clamp utilizing a potassium gluconate based intracellular solution. Inset: Response of the same cell under voltage clamp to a change in membrane potential from -80 mV to -10 mV elicited a peak current of 457 pA. Scale bars for inset: 5 ms, 0.2 nA.