p-Fect

p-Fect provides the following advantages and benefits: 

• New, unique formulation
• Highest transfection efficiency for many different cell lines:
  •  Hela, HeLa-S3, COS-1, COS-7, Hep-G2, NIH-3T3, P19-PC-12, RAW267.4 and more!
• Low cytotoxicity
• Simple, fast and straightforward protocol

Image: RAW267.4 cells (a murine macrophage cell line) were grown to 60% confluency. The following mixture was then used for transfection: a pGeneGrip vector that encodes GFP and is labeled with rhodamine (8 ug), DNA diluent A (168 ul), and GenePORTER 2 transfection reagent (40ul). Twenty four hours post transfecton, cells were examined using fluorescence microscopy. Panel A shows about 80% of the cells were transfected with the pGeneGrip vector. Panel B shows that about 50% of the cells were positive in GFP expression.

Please note:

• pn-Fect™-delivers plasmids to primary Neurons.
•  i-Fect ™ is designed for delivering siRNA and DsiRNA to Primary Cells.