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The Strep-tag purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. This technology allows one-step purification of almost any recombinant protein under physiological conditions, thus preserving its bioactivity. The Strep-tag system can be used to purify Strep-tag II proteins from any expression system including baculovirus, mammalian cells, yeast, and bacteria. For vectors for bacterial expression. Cloning and expression kits and vectors.

Capabilities:
  • Simple one-step purification from crude lysate to 99% pure protein under physiological conditions possible.
  • High and selective binding affinity and high capacity due to special Strep-Tactin resins
  • Resins for gravity flow, low pressure or HPLC applications
  • Co-purification of non-covalently bound ligands, thus, protein-protein interaction studies are possible
  • Column regeneration and activity status is visualized by color change.
  • Testimonial:  "We are very happy with the Strep-Tag system.  I  recently presented my protein purification data, as well as a cost comparison between a typical  flag resin purification  (our usual purification tag of   choice) and the StrepII tag purification and everyone was impressed.   We do A LOT of protein purification with flag resin, so we are happy to see that the StrepII tag works equally well at a fraction of the cost.  Any new constructs I build will now contain the StrepII tag, and I know others in the lab who will be building StrepII tags into their constructs as well." Michele Jaeger, Dr. Ervasti Lab, University of Minnesota.

    Brought to you by IBA

      
    Strep-tag® Protein Purification-Strep-tactin Columns, Resins and Related Reagents
    Strep-tactins sytems for easy and fast Strep-tag protein purifcations
    Strep-tag® Detection Kits, Antibodies and Conjugates
    Designed for Western Blotting, Immune fluorescence, FACS, Detection in ELISA
    Strep-Tactin Resin Specifications
    Specifications for Strep-Tactin Sepharose®, Strep-Tactin Superflow® and Strep-Tactin Macroprep®.
    Strep-Tactin Column-Resin Comparison Table
    Linear Flow Calculations
    Convert between Linear and Volumetric
    Strep-tag® Related Content
    Detailed information on IBA's Strep-Tag Products