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Applying methods and neural markers for sorting of hESC-derived differentiated neuronal cell types: FACS for NCAM for in vitro and in vivo proof-of-principle studies. (a) NCAM was upregulated upon neural differentiation in vitro and clusters of immature, Oct-4+ cells did not express NCAM, allowing for positive selection strategies to eliminate unwanted immature stem cells.
Image: Nuclei of D3 or IGnT-KO ES cells stained with goat anti-Oct3/4 antibody. doi: 10.1093/glycob/cwm138
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| Applying methods and neural markers for sorting of hESC-derived differentiated neuronal www.StemCells.com by Ole Isacson on June 22, 2007 | |