ASIC3

Yoshiyasu Uchiyama, Chin-Chang Cheng, Keith G Danielson, Joji Mochida, Todd J Albert, Irving M Shapiro and and Makarand V Risbud. Expression of Acid-Sensing Ion Channel 3 (ASIC3) in Nucleus. Pulposus Cells of the Intervertebral Disc Is Regulated by p75NTR and ERK Signaling. JOURNAL OF BONE AND MINERAL RESEARCH. Volume 22, Number 12, 2007 Published online on August 13, 2007; doi: 10.1359/JBMR.070805.© 2007 American Society for Bone and Mineral Research ... 

...Freshly isolated discs from adult rats were immediately fixed in 4% paraformaldehyde in PBS, whereas whole mouse embryos (E15.5) were perfusion fixed. Discs or embryos were embedded in paraffin. Transverse and coronal sections, 6–8 um in thickness, were deparaffinized in xylene, rehydrated through graded ethanol, and stained with Alcian blue and with eosin and propidium iodide. For localizing ASIC3, sections were incubated with the anti- ASIC3 antibody (Neuromics) in 2% BSA in PBS at a dilution of 1:100 at 4°C overnight. After thoroughly washing the sections, the bound primary antibody was incubated with biotinylated universal secondary antibody at a dilution of 1:20 (Vector Laboratories) for 10 min at room temperature. Sections were incubated with a streptavidin/peroxidase complex for 5 min and washed with PBS, and color was developed using 3
-3-diaminobenzidine (Vecta Stain Universal Quick Kit; Vector Laboratories). Negative control for staining included use of isotype IgG (1:100)... 

R. Ambalavanara, C. Yallampallib, U. Yallampallib and D. Dessema. Injection of adjuvant but not acidic saline into craniofacial muscle evokes nociceptive behaviors and neuropeptide expression. doi:10.1016/j.neuroscience.2007.07.058.

...guinea-pig polyclonal antibody against ASIC3 (1:1000, Neuromics, USA)...

T. Fukuda,  H. Ichikawa, R. Terayama, T. Yamaai, T. Kuboki and T. Sugimoto. ASIC3-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia. doi:10.1016/j.brainres.2006.01.039

...The sections were incubated with guinea-pig anti-ASIC3 serum (1:200, Neuromics)...